Evaluation of Antioxidant Enzyme Levels of Glutathione Peroxidase in Patients Undergoing A Autologous Hematopoietic STEM CELL

The Autologous Hematopoietic Stem Cell Transplantation (AHSCT) is a therapeutic method used in various immunological, hematological and genetic disorders, which uses patient's own stem cells collected from peripheral blood after their (the stem cells) mobilization by granulocyte colony-stimulating factors (GCS-F), in order to restore the damaged or defective bone marrow function. Oxidative stress and antioxidant depletion have been described during the stages involved in AHSCT. Glutathione peroxidase is an enzyme responsible for detoxification of organic and inorganic peroxides, which participates of enzymatic cellular antioxidant defense. The aim of the study was to evaluate the levels of glutathione peroxidase in various stages involving AHSCT in patients undergoing autologous hematopoietic stem cell transplantation. Glutathione peroxidase was determined using the Glutathione Peroxidase Ransel^® kit (Randox). We measured glutathione peroxidase levels of eight patients at different times pre-and post-AHSCT: before Conditioning Regimen (CR), 24 hours after chemotherapy, 10 days (D +10) and 20 days (D +20) after transplantation. There was a statistically significant difference in levels of glutathione peroxidase after CR comparing to other times, suggesting an increase in oxidative stress after chemotherapy. According to previous studies, oxidative stress occurs acutely after high-dose chemotherapy for AHSCT and is apparently related to late hematological recovery. Thus, it can be suggested the use of glutathione peroxidase levels, and other oxidative stress parameters as toxicity and grafting markers after AHSCT.

Keyword: Bone Marrow Transplantation; Transplantation, Autologous; Glutathione Peroxidase.