Methods: Twenty-three patients median age 33 yrs (range 4 - 69 yrs) received a CB transplant (CBT) as a second allogeneic HCT at the Fred Hutchinson Cancer Research Center between 2006 – 2013. Indication for second allograft were relapse (n = 19), graft failure (n = 3) and donor derived MDS (n = 1). Median time between transplants was 505 days (range, 55 - 3515). At the time of second HCT, 19 patients were in CR and 4 patients had persistent disease. For their second transplant, 10 patients received reduced intensity conditioning (RIC) with fludarabine (Flu) 200mg/m2, cyclophosphamide (Cy) 50 mg/kg and 200 - 400 cGy TBI, 2 patients received myeloablative (MAC) conditioning with Flu 75mg/m2, Cy 120mg/kg and 1200 - 1320cGy TBI (n = 2), and 11 patients received “midi” conditioning with treosulfan 42mg/kg, Flu 150 - 200mg/m2 and 200 cGy TBI. All patients received GVHD prophylaxis with cyclosporine and mycophenolate mofeteil. Twenty of the 23 patients received a double CBT with a median TNC of 4.8 x 107 cells/kg and a median CD34+ of 2.2 x 105 cells/kg. Twelve patients received at least one 4/6 graft; 9 patients received at least one 5/6 graft.
Results: Median time to engraftment was 22 days (range day 6 - 49) among 21 evaluable patients; 2 patients died prior to engraftment. Median time to platelet engraftment was 55 days (range 23 - 83). Day +28 CD3+ chimerism was 100% second transplant donor in 16 of 19 evaluable patients, and 100% first donor in 2. One patient was mixed between first (22%) and second donor (78%). Death prior to Day 100 was seen in 5 patients (infection/organ failure (n = 3), encephalopathy (n = 1), and relapse (n = 1). Acute grade II-IV and III-IV GVHD was diagnosed in 18 of 21 and 6 of 21 evaluable patients respectively, and 9 of 16 evaluable patients developed chronic GVHD (6 mild, 2 moderate, 1 severe). Disease free survival, treatment related mortality, and relapse at 2 years was 40%, 33%, and 35% respectively. Patients receiving MAC/midi vs RIC conditioning experienced a 2-year DFS of 46 vs 15% (p = 0.02) and relapse rate of 26 vs 45% (p = 0.20).
Conclusions: Our results demonstrate quite favorable outcomes in patients undergoing CBT as a second allogeneic HCT, with all evaluable patients engrafting and full donor chimerism achieved in 16 of 19 patients by Day +28. The use of CB as the stem cell source is particularly attractive in this setting as it eliminates the need to put a conventional donor at risk.
Novartis , advisor: chair, data safety monitoring board
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