Introduction: Reduced intensity conditioning (RIC) regimens decrease early toxicity after allogeneic hematopoietic cell transplant (HCT), but their ultimate success depends on establishing a graft-versus-tumor effect (GVT). Because graft-versus-host disease (GVHD) and GVT are tightly linked, complete elimination of GVHD may lead to an unintended increased relapse risk in a RIC setting. Therefore, we tested a novel GVHD prophylaxis regimen intended to preserve GVT but minimize GVHD mortality.
Methods: 48 patients undergoing RIC unrelated donor transplant (URD HCT) enrolled from 2009-2012 on a phase II trial that added etanercept and extracorporeal photopheresis (ECP) to a standard GVHD prophylaxis of tacrolimus and mycophenolate mofetil (MMF). The preferred RIC was fludarabine 160 mg/m2 + busulfan 6.4-12.8 mg/kg +/- TBI 200 cGy. GVHD prophylaxis consisted of overlapping agents as follows: Tacrolimus from d-3 to d56 (then tapered over 4 months in the absence of acute GVHD), MMF d0-28, etanercept 25 mg subcutaneously 2x weekly from d0-56 (16 doses), and ECP beginning on d28. ECP was given once weekly from d28-70, then every other week until d100, then monthly until d180 for a total of 12 treatments. Donors were required to match to recipients for 7-8/8 HLA loci.
Results: The median age of the study patients was 60 yr (18-71). Donors were 7/8 (n=14, 29%) or 8/8 (n=34, 71%) matched. Patients engrafted at a median of 12d (8-26d). The 12mo post-HCT end points for the entire study cohort were as follows: overall survival 73% (95% CI 61-87) (Figure 1A), non-relapse mortality (NRM) 21% (9-32), and relapse of 19% (8-30). The cumulative incidence of grade II-IV acute GVHD at d100 and 6mo was 46% (32-60) and 57% (42-71) respectively, and for grade III-IV acute GVHD was 17% (6-27) and 19% (8-30). In an exploratory analysis (Figure 1B), GVHD that developed after 8 doses of etanercept and at least one ECP treatment (d28) was significantly less lethal than GVHD that developed before ECP began (6mo NRM 11% vs 40%, 12 mo 21% vs 50%; p=0.04), which suggests that ECP prophylaxis may ameliorate GVHD lethality. The possible benefit of prophylactic ECP on GVHD outcomes does not appear to be related to delayed GVHD onset as NRM did not differ in control patients diagnosed before or after d28. In 69 contemporaneous control patients with grade 2-4 GVHD, 6mo and 12 mo NRM was 32% and 39% respectively, and there was no difference between patients that developed GVHD after or before d28 (6 mo 27% vs 36%, 12 mo 33% vs 44%; p=0.57).
Conclusions: Six month (83%) and 1yr survival (73%) was excellent in older, frequently mismatched URD HCT patients, even though GVHD incidence was not lowered. An exploratory analysis suggested that if prophylactic ECP has benefit, the effect is confined to patients who start ECP before GVHD develops. Strategies focusing on earlier delivery of ECP to URD HCT patients could be explored in future studies.
Merck, Inc, None: Advisory Board