411 Donor Cell Cardiac Myeloid Sarcoma Following Umbilical Cord Transplantation

Track: Poster Abstracts
Saturday, February 14, 2015, 6:45 PM-7:45 PM
Grand Hall CD (Manchester Grand Hyatt)
Christi Ann Hayes, MD , Bone Marrow and Stem Cell Transplantation, Icahn School of Medicine at Mount Sinai, New York, NY
Luis Isola, MD , Bone Marrow and Stem Cell Transplantation, Icahn School of Medicine at Mount Sinai, New York, NY
Eileen Scigliano, MD , Bone Marrow and Stem Cell Transplantation, Icahn School of Medicine at Mount Sinai, New York, NY
Keren Osman, MD , Bone Marrow and Stem Cell Transplantation, Icahn School of Medicine at Mount Sinai, New York, NY
Amir Steinberg, MD , Bone Marrow and Stem Cell Transplantation, Icahn School of Medicine at Mount Sinai, New York, NY
Alla Keyzner, MD , Bone Marrow and Stem Cell Transplantation, Icahn School of Medicine at Mount Sinai, New York, NY
Anne S. Renteria, MD , Bone Marrow and Stem Cell Transplantation, Icahn School of Medicine at Mount Sinai, New York, NY
Bruce Petersen, MD , Department of Pathology, Mount Sinai Medical Center, New York, NY
Adriana K. Malone, MD , Bone Marrow and Stem Cell Transplantation, Icahn School of Medicine at Mount Sinai, New York, NY
Presentation recording not available for download or distribution as requested by the presenting author.
Background

Donor cell leukemia (DCL) is a rare complication of transplantation. In 1971, Fialkow reported the case of a female patient with ALL who relapsed after a transplant from her brother. Cytogenetics showed only donor derived  XY metaphases. Sporadic cases of DCL have since been reported, however, the precise incidence remains unclear. An EBMT review of 10489 allogeneic transplants found an incidence of ~0.1%.

Case Report

A 41-year-old-man was admitted 567 days after a double unrelated blood cord transplantation (UBCT) for B-ALL.  He received reduced-intensity conditioning prior to his UBCT with cyclophosphamide 50 mg/kg, fludarabine 200 mg/m2, and 200 cGy TBI (Cy/Flu/TBI). GVHD prophylaxis included CSA and MMF. Post-transplant, bone marrow (BM) and peripheral blood (PB) chimerism was 100% donor.

 On day+515 post-transplant he was thrombocytopenic with 8% blasts. A BM biopsy revealed a donor derived AML, with 22% blasts. BM chimerism remained 100% donor.  He received decitabine at 20mg/m2 for 10 days. Post- treatment BM biopsy was negative for AML.

 On day+567, he was admitted with fever, pleuritic chest pain and tachycardia. EKG demonstrated T-wave inversions in V3-V5. Both troponin (1.8 ng/ml) and BNP (805 pg/ml) were elevated. Chest CT revealed a circumferential pericardial and nodular soft tissue densities posterior to the heart and he ultimately underwent a pericardectomy.

 The pericardial pathology showed myeloid sarcoma. The tumor cells were CD43+, MPO focal+, CD34 focal+, CD117 focal+, CD68 weak+, CD163 focal+, PAX5-, CD56-, TdT-, CD79a focal weak+ by immunohistochemistry. The chimerism revealed that the lesion was entirely donor derived.  A BM biopsy showed no evidence of AML with normal hematopoiesis and full donor chimerism. Thus, cardiac myeloid sarcoma represented an isolated site of disease.

Unfortunately, the patient continued to deteriorate and expired on day+579.

 Discussion

Within DCL, isolated donor derived granulocytic sarcoma, as in our patient, is an extremely rare and challenging diagnosis. Reactive donor leukocytes can infiltrate a host derived granulocytic sarcoma making it difficult to distinguish relapse from a DCL.

Given the comparatively recent introduction of UCBT, fewer cases of cord blood associated DCL leukemia have been reported in the literature when compared with PBSCT or BMT. Some interesting trends have emerged. AML is the most common type of UCBT DCL.  There is a shorter interval to the development of DCL in UCBT (median of 14.5 months) compared to BMT or PBSCT (36 months). Abnormal karyotypes, particularly monosomy 7, are more common in UCBT DCL. Perhaps, UCB is uniquely sensitive to leukemogenic effects of conditioning. Given the predominance of AML DCL in UCBT, more cases of donor myeloid sarcoma, may emerge. Improved molecular testing will allow precise detection and avoid mistakenly categorizing DCL as relapsed disease.

Disclosures:
B. Petersen, Incyte , Hematopathology Publication Steering Committee: Consultancy