Methods: We conducted a retrospective analysis of 78 patients who underwent ASCT at the Mayo Clinic 1/1/2008 - 12/21/2010. Patients with unrelated volunteer donors were matched for HLA-A, B, C, DRB1 and DQB1 loci (10/10) at the allele level without consideration of the HLA-DPB1 locus. Patient and donor HLA-DPB1 genotyping was performed by reverse SSO typing and the ImMunoGeneTics/HLA (IMGT/HLA) T-cell epitope matching algorithm was used to determine mismatch status as permissive or non-permissive.
Results: Of 78 donor recipient pairs, there were 13 HLA-DPB1 matches, 42 permissive mismatches, and 23 non-permissive mismatches (10 in the graft-vs-host [GvH] direction, 13 in the host-vs-graft [HvG] direction). In a univariate analysis, when matches and permissive mismatches were combined into a group and compared to non-permissive mismatch, there was no significant difference in overall survival (p=0.31), relapse (p=0.79), or neutrophil or platelet engraftment (p=0.23 and p=0.99, respectively). No significant difference was observed in acute graft-vs-host disease (aGVHD; p=0.25) or grade II-IV aGVHD (p=0.62) among the matched, permissive mismatched, and non-permissive mismatched groups. The permissive mismatched group showed an increase in chronic GVHD (cGVHD) over the matched and the non-permissive mismatched groups that neared significance (p=0.052).
In univariate analyses, for non-permissive mismatches, theGvH mismatch direction trended toward reduced survival (p=0.26), increased relapse (p=0.13), increased aGVHD (p=0.064), and increased cGVHD (p=0.22).
Conclusions: We did not identify any significant differences in survival, relapse, engraftement, or acute or chronic GVHD among the patients with HLA-DPB1 donor matches, permissive mismatches, and non-permissive mismatches, although our study was limited by small sample size. Further work from large databases is necessary to fully understand the impact on clinical outcomes of HLA-DPB1 mismatches.